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Human IL-36 Beta/IL-1F8 ELISA Kit

Toimittaja: ANTIBODIES.COM
Human IL-36 Beta/IL-1F8 ELISA Kit

A312158-96
ANTIA312158-96EA 545 EUR
ANTIA312158-96
Human IL-36 Beta/IL-1F8 ELISA Kit
Bio-assayt ELISA-kitit

Human IL-36 beta/IL-1F8 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-36 beta/IL-1F8 in serum, plasma, and other biological fluids.


  • Higher throughput: Get results in just 90 minutess, with a single wash step
  • Detection range: 5 to 80 ng/L
  • Sensitivity: 0,24 ng/L
  • Sample Type: Serum, plasma or other biological fluids
  • Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%


Human IL-36 beta/IL-1F8 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human IL-36 beta/IL-1F8 in serum, plasma or other biological fluids. An antibody specific for IL-36 beta/IL-1F8 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-36 beta/IL-1F8 present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-IL-36 beta/IL-1F8 antibody, which also binds the IL-36 beta/IL-1F8 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-IL-36 beta/IL-1F8 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-IL-36 beta/IL-1F8 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of IL-36 beta/IL-1F8 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of IL-36 beta/IL-1F8 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.


Tilaustiedot: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, steptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.

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