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Human BRK/PTK6 ELISA Kit

Toimittaja: ANTIBODIES.COM
Human BRK/PTK6 ELISA Kit

A311219-96
ANTIA311219-96EA 545 EUR
ANTIA311219-96
Human BRK/PTK6 ELISA Kit
Bio-assayt ELISA-kitit

Human Brk/PTK6 ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Brk/PTK6 in serum, plasma, and other biological fluids.


  • Higher throughput: Get results in just 90 minutes, with a single wash step
  • Assay precision: Intra assay: CV <8%, Inter assay: CV <10%


Human Brk/PTK6 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Brk/PTK6 in serum, plasma or other biological fluids. An antibody specific for Brk/PTK6 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Brk/PTK6 present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-Brk/PTK6 antibody, which also binds the Brk/PTK6 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Brk/PTK6 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Brk/PTK6 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. colour develops in proportion to the amount of Brk/PTK6 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Brk/PTK6 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.


Tilaustiedot: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, positive control, negative control, HRP-conjugated detection antibody, sample diluent, substrate solution A, substrate solution B, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.

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